Preprints

More to come!

Accepted Publications

SUNY Upstate Medical University

Wolfe, S. A., Ma, Y., Yaron-Barir, T. M., Chang, C., Pilo, C. A., Ghassemian, M., Roberts, A. J., Lee, S. R., Henson, B. A., Jepsen, K., Johnson, J. L., Cantley, L. C., Taylor, S. S., Gorrie, G. and Newton, A. C. “Sex specific disruptions in PKCgamma signaling in a mouse model of Spinocerebellar Ataxia Type 14.” JCI Insight (2026). https://doi.org/10.1172/jci.insight.192155. (PMID: 41926327) (Pubmed)

Mayfield, J. E., Wang, J., Tovell, H., Taylor, S. S., Gonias, S. L. and Sigurdson, C. J. “Cellular Prion Protein Engages the N-Methyl-d-Aspartate Receptor through N- and C-Terminal Domains.” Biochemistry (2026). https://doi.org/10.1021/acs.biochem.6c00011. PMID: 41860118 (Pubmed)

University of California - San Diego

Gauron, M. C., Prokopenko, D., Lee, S., Wolfe, S. A., Hecker, J., Willett, J., Waqas, M., Lordén, G., Yang, Y., Mayfield, J. E., Castanho, I., Mullin, K., Morgan, S., Hahn, G., Demeo, D. L., Hide, W., Bertram, L., Lange, C., Newton, A. C. and Tanzi, R. E. “A PKCη missense mutation enhances Golgi-localized signaling and is associated with recessively inherited familial Alzheimer’s disease.” Sci Signal (2025). https://doi.org/10.1126/scisignal.adv0970. (PMID: 40591711) (Pubmed)

Mayfield, J. E.&, Rajic, A. J.&, Aguilar-Calvo, P., Soldau, K., Flores, S., Lawrence, R., Choudhury, B., Ghassemian, M., Pizzo, D. P., Wagner, S. L., Danque, G. A., Sumowski, P., Hansen, L. A., Goodwill, V., Esko, J. D. & Sigurdson, C. J. Multi-omic analysis of meningeal cerebral amyloid angiopathy reveals enrichment of unsubstituted glucosamine and extracellular proteins. J Neuropathol Exp Neurol (2025). https://doi.org/10.1093/jnen/nlaf018. PMID: 40156913 (Pubmed)

Lin, J.&, Callender, J. A.&, Mayfield, J. E.&, McClatchy, D. B., Ojeda-Juarez, D., Pourhamzeh, M., Soldau, K., Kurt, T. D., Danque, G. A., Khuu, H. K., Ronson, J. E., Pizzo, D. P., Du, Y., Gruber, M. A., Sevillano, A. M., Wang, J., Orru, C. D., Chen, J., Funk, G., Aguilar-Calvo, P., Aulston, B. D., Roy, S., Rho, J. M., Bui, J. D., Newton, A. C., Lipton, S. A., Caughey, B., Patrick, G. N., Dore, K., Yates Iii, J. R. & Sigurdson, C. J. Mutant prion protein enhances NMDA receptor activity, activates PKC, and triggers rapid excitotoxicity in mice. J Clin Invest (2025). https://doi.org/10.1172/JCI186432. PMID: 40185484 (Pubmed)

Mayfield, J. E. & Dixon, J. E. Emerging mechanisms of regulation for endoplasmic/sarcoplasmic reticulum Ca2+ stores by secretory pathway kinase FAM20C. Curr Opin Chem Biol 74, 102279 (2023). https://doi.org/10.1016/j.cbpa.2023.102279. PMID: 36966700 (Pubmed)

Gu, L., Zhu, Y., Watari, K., Lee, M., Liu, J., Perez, S., Thai, M., Mayfield, J. E., Zhang, B., Cunha, E. R. K., Li, F., Kim, L. C., Jones, A. C., Wierzbicki, I. H., Liu, X., Newton, A. C., Kisseleva, T., Lee, J. H., Ying, W., Gonzalez, D. J., Saltiel, A. R., Simon, M. C. & Karin, M. Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness. Cell Metab 35, 1009-1021 e1009 (2023). https://doi.org/10.1016/j.cmet.2023.03.021. PMID: 37084733 (Pubmed)

Li, F. L., Fu, V., Liu, G., Tang, T., Konradi, A. W., Peng, X., Kemper, E., Cravatt, B. F., Franklin, J. M., Wu, Z., Mayfield, J., Dixon, J. E., Gerwick, W. H. & Guan, K. L. Hippo pathway regulation by phosphatidylinositol transfer protein and phosphoinositides. Nat Chem Biol 18, 1076-1086 (2022). https://doi.org/10.1038/s41589-022-01061-z. PMID: 35788180 (Pubmed)

Worby, C. A., Mayfield, J. E., Pollak, A. J., Dixon, J. E. & Banerjee, S. The ABCs of the atypical Fam20 secretory pathway kinases. J Biol Chem 296, 100267 (2021). https://doi.org/10.1016/j.jbc.2021.100267. PMID: 33759783 (Pubmed)

Rios, J. J., Denton, K., Russell, J., Kozlitina, J., Ferreira, C. R., Lewanda, A. F., Mayfield, J. E., Moresco, E., Ludwig, S., Tang, M., Li, X., Lyon, S., Khanshour, A., Paria, N., Khalid, A., Li, Y., Xie, X., Feng, J. Q., Xu, Q., Lu, Y., Hammer, R. E., Wise, C. A. & Beutler, B. Germline Saturation Mutagenesis Induces Skeletal Phenotypes in Mice. J Bone Miner Res 36, 1548-1565 (2021). https://doi.org/10.1002/jbmr.4323. PMID: 33905568 (Pubmed)

Banerjee, S., Wei, T., Wang, J., Lee, J. J., Gutierrez, H. L., Chapman, O., Wiley, S. E., Mayfield, J. E., Tandon, V., Juarez, E. F., Chavez, L., Liang, R., Sah, R. L., Costello, C., Mesirov, J. P., de la Vega, L., Cooper, K. L., Dixon, J. E., Xiao, J. & Lei, X. Inhibition of dual-specificity tyrosine phosphorylation-regulated kinase 2 perturbs 26S proteasome-addicted neoplastic progression. Proc Natl Acad Sci U S A 116, 24881-24891 (2019). https://doi.org/10.1073/pnas.1912033116. PMID: 31754034 (Pubmed)

Pollak, A. J., Liu, C., Gudlur, A., Mayfield, J. E., Dalton, N. D., Gu, Y., Chen, J., Heller Brown, J., Hogan, P. G., Wiley, S. E., Peterson, K. L. & Dixon, J. E. A secretory pathway kinase regulates sarcoplasmic reticulum Ca(2+) homeostasis and protects against heart failure. Elife 7 (2018). https://doi.org/10.7554/eLife.41378. PMID: 30520731 (Pubmed)

Banerjee, S., Ji, C., Mayfield, J. E., Goel, A., Xiao, J., Dixon, J. E. & Guo, X. Ancient drug curcumin impedes 26S proteasome activity by direct inhibition of dual-specificity tyrosine-regulated kinase 2. Proc Natl Acad Sci U S A 115, 8155-8160 (2018). https://doi.org/10.1073/pnas.1806797115. PMID: 29987021 (Pubmed)

University of Texas at Austin

Zhang, Q., Kim, W., Panina, S. B., Mayfield, J. E., Portz, B. & Zhang, Y. J. Variation of C-terminal domain governs RNA polymerase II genomic locations and alternative splicing in eukaryotic transcription. Nat Commun 15, 7985 (2024). https://doi.org/10.1038/s41467-024-52391-6. PMID: 39266551 (Pubmed)

Gong, S., Kirmizialtin, S., Chang, A., Mayfield, J. E., Zhang, Y. J. & Johnson, K. A. Kinetic and thermodynamic analysis defines roles for two metal ions in DNA polymerase specificity and catalysis. J Biol Chem 296, 100184 (2021). https://doi.org/10.1074/jbc.RA120.016489. PMID: 33310704 (Pubmed)

Ramani, M. K. V., Escobar, E. E., Irani, S., Mayfield, J. E., Moreno, R. Y., Butalewicz, J. P., Cotham, V. C., Wu, H., Tadros, M., Brodbelt, J. S. & Zhang, Y. J. Structural Motifs for CTD Kinase Specificity on RNA Polymerase II during Eukaryotic Transcription. ACS Chem Biol 15, 2259-2272 (2020). https://doi.org/10.1021/acschembio.0c00474. PMID: 32568517 (Pubmed)

Mayfield, J. E.#, Irani, S. & Zhang, Y#. Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates. Bio Protoc 10, e3648 (2020). https://doi.org/10.21769/BioProtoc.3648. PMID: 33659319 (Pubmed)

Wolfe, S. A., Farris, S. P., Mayfield, J. E., Heaney, C. F., Erickson, E. K., Harris, R. A., Mayfield, R. D. & Raab-Graham, K. F. Ethanol and a rapid-acting antidepressant produce overlapping changes in exon expression in the synaptic transcriptome. Neuropharmacology 146, 289-299 (2019). https://doi.org/10.1016/j.neuropharm.2018.11.007. PMID: 30419244 (Pubmed)

Mayfield, J. E.&, Irani, S.&, Escobar, E. E., Zhang, Z., Burkholder, N. T., Robinson, M. R., Mehaffey, M. R., Sipe, S. N., Yang, W., Prescott, N. A., Kathuria, K. R., Liu, Z., Brodbelt, J. S. & Zhang, Y. Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb. Elife 8 (2019). https://doi.org/10.7554/eLife.48725. PMID: 31385803 (Pubmed)

Burkholder, N. T.&, Mayfield, J. E.&, Yu, X., Irani, S., Arce, D. K., Jiang, F., Matthews, W. L., Xue, Y. & Zhang, Y. J. Phosphatase activity of small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST). J Biol Chem 293, 16851-16861 (2018). https://doi.org/10.1074/jbc.RA118.004722. PMID: 30217818 (Pubmed)

Portz, B., Lu, F., Gibbs, E. B., Mayfield, J. E., Rachel Mehaffey, M., Zhang, Y. J., Brodbelt, J. S., Showalter, S. A. & Gilmour, D. S. Structural heterogeneity in the intrinsically disordered RNA polymerase II C-terminal domain. Nat Commun 8, 15231 (2017). https://doi.org/10.1038/ncomms15231. PMID: 28497792 (Pubmed)

Mayfield, J. E.&, Robinson, M. R.&, Cotham, V. C., Irani, S., Matthews, W. L., Ram, A., Gilmour, D. S., Cannon, J. R., Zhang, Y. J. & Brodbelt, J. S. Mapping the Phosphorylation Pattern of Drosophila melanogaster RNA Polymerase II Carboxyl-Terminal Domain Using Ultraviolet Photodissociation Mass Spectrometry. ACS Chem Biol 12, 153-162 (2017). https://doi.org/10.1021/acschembio.6b00729. PMID: 28103682 (Pubmed)

Mayfield, J. E.&, Burkholder, N. T.& & Zhang, Y. J. Dephosphorylating eukaryotic RNA polymerase II. Biochim Biophys Acta 1864, 372-387 (2016). https://doi.org/10.1016/j.bbapap.2016.01.007. PMID: 26779935 (Pubmed)

Irani, S., Yogesha, S. D., Mayfield, J., Zhang, M., Zhang, Y., Matthews, W. L., Nie, G., Prescott, N. A. & Zhang, Y. J. Structure of Saccharomyces cerevisiae Rtr1 reveals an active site for an atypical phosphatase. Sci Signal 9, ra24 (2016). https://doi.org/10.1126/scisignal.aad4805. PMID: 26933063 (Pubmed)

Mayfield, J. E., Fan, S., Wei, S., Zhang, M., Li, B., Ellington, A. D., Etzkorn, F. A. & Zhang, Y. J. Chemical Tools To Decipher Regulation of Phosphatases by Proline Isomerization on Eukaryotic RNA Polymerase II. ACS Chem Biol 10, 2405-2414 (2015). https://doi.org/10.1021/acschembio.5b00296. PMID: 26332362 (Pubmed)

Yogesha, S. D., Mayfield, J. E. & Zhang, Y. Cross-talk of phosphorylation and prolyl isomerization of the C-terminal domain of RNA Polymerase II. Molecules 19, 1481-1511 (2014). https://doi.org/10.3390/molecules19021481. PMID: 24473209 (Pubmed)

Zhang, M., Yogesha, S. D., Mayfield, J. E., Gill, G. N. & Zhang, Y. Viewing serine/threonine protein phosphatases through the eyes of drug designers. FEBS J 280, 4739-4760 (2013). https://doi.org/10.1111/febs.12481. PMID: 23937612 (Pubmed)

New York University

Pick, J. E., Wang, L., Mayfield, J. E. & Klann, E. Neuronal expression of the ubiquitin E3 ligase APC/C-Cdh1 during development is required for long-term potentiation, behavioral flexibility, and extinction. Neurobiol Learn Mem 100, 25-31 (2013). https://doi.org/10.1016/j.nlm.2012.11.005. PMID: 23238556 (Pubmed)